I-9: Embryo Assessment and Selection
Authors
Abstract:
Over the years it has been an important goal to optimize the treatment of subfertility in order to obtain a better pregnancy rate and baby-take-home rate, usually combined with the goal of decreasing the rate of multiplet pregnancies. This implies optimizing the development of embryos through improved in vitro culture methods. And it also implies optimizing the methods of selection of embryos for fresh and frozen transfers, as well as the timing and location of the deposition of the embryos during transfer. Non-invasive techniques for assessment of implantation potential of embryos can improve the success rate and hence be of importance not least when transferring single embryos. Using time lapse investigations it is possible to follow the morphological development without interfering with the cell culture processes and the natural cell divisions. Important parameters are e.g. morphology, cleavage pattern and fragmentation. This gives the possibility of evaluating the embryo’s chance for survival and implantation - and ultimately for developing into a child. An alternative method of improving this evaluation is metabolomic profiling. The type and amount of metabolites used and secreted by the embryo during culture can give an indication of the possibilities for survival and further fetal development. This profiling is carried out in the culture medium after transfer of the embryos to another dish - with other words without interference with the culture processes
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Journal title
volume 7 issue 3
pages 5- 5
publication date 2013-09-01
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